Journal: PLoS Pathogens

Article Title: Protective effects of combining monoclonal antibodies and vaccines against the Plasmodium falciparum circumsporozoite protein

doi: 10.1371/journal.ppat.1010133

Figure Lengend Snippet: (A) MFI of Pb-PfCSP-SPZ bound by 0.2 μg/mL CIS43-, L9-, or 317-AF750 when co-incubated with PBS + 10% FBS (PBS-FBS) or 1:5 diluted serum pooled from either one naïve US adult volunteer, ten naïve mice immunized 3x with 1 μg R21 + adjuvant, fifteen naïve US adults immunized 3x with 9x10 5 irradiated PfSPZ, and ten Malian children/adults naturally exposed to malaria. For each PfCSP repeat mAb, P- values were determined by comparing each serum type to the PBS-FBS control using a two-way ANOVA with Bonferroni’s post-hoc correction. (B) Endpoint titers of pooled serum from A binding to FL-rCSP measured by ELISA. P- values were determined by comparing serum types to naïve serum using the Kruskal-Wallis test. (C) R21 immunization scheme in normal mice, which received two intramuscular injections of 1 μg R21 + adjuvant at three week intervals prior to passive transfer of PfCSP repeat mAbs two hours before IV challenge with 2,000 Pb-PfCSP-SPZ. (D-F) Liver burden 40 hours after IV challenge with 2,000 Pb-PfCSP-SPZ in mice (n = 5-20/group; 50μg/25μg data in E/F were combined from two experiments, circles and squares) immunized with 1 μg R21 alone; mice administered CIS43, L9, and 317 alone (D, 150 μg; E, 50 μg; F, 25 μg); and mice immunized with 1 μg R21 and administered CIS43, L9, and 317 (D, 150 μg; E, 50 μg; F, 25 μg). P- values were determined by comparing each R21 + mAb combination to the R21 alone or respective mAb alone groups using the Kruskal-Wallis test. (A-B, D-F): ***, p<0.001; **, p<0.01; *, p<0.05; ns (not significant), p>0.05.

Article Snippet: R21 immunogen [ ] was diluted in sterile PBS to 1 μg with 33.3 μL of army liposomal formula Q (ALFQ; liposomal adjuvant formulation containing monophosphoryl lipid A and QS-21 [ ]) in a final volume of 50 μL.

Techniques: Incubation, Adjuvant, Irradiation, Control, Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Growth Differentiation Factor‐15 Deficiency Inhibits Atherosclerosis Progression by Regulating Interleukin‐6–Dependent Inflammatory Response to Vascular Injury

doi: 10.1161/JAHA.112.002550

Figure Lengend Snippet: Oligonucleotides Used for Genotyping Polymerase Chain Reaction

Article Snippet: R21 reverse , 5′‐GGC CAC CAG GTC ATC ATA AG‐3′ , , Metabion, Planegg‐Martinsried, Germany.

Techniques: Sequencing, Amplification, Knock-Out